ENDONUCLEASE BSTI Meaning and
Definition
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Endonuclease BstI is a type II restriction enzyme that recognizes a specific DNA sequence and cleaves it at a particular point. It is isolated from the bacterium Bacillus stearothermophilus and belongs to the restriction modification system BstI, hence its name. This enzyme plays a crucial role in molecular biology research and genetic engineering techniques.
The BstI endonuclease recognizes a specific DNA sequence known as the BstI recognition site or restriction site. This site is composed of two inverted palindromic sequences, typically 6 nucleotides long, and has the sequence 5'-GGATG(N)4C-3' (where N represents any nucleotide). Once the enzyme recognizes this specific sequence, it cleaves the DNA strand within the recognition site, producing two fragments with sticky ends. The sticky ends generated can then be used for further DNA manipulations such as cloning, gene mapping, or DNA sequencing.
Endonuclease BstI shows excellent specificity and efficiency in recognizing and cleaving its target DNA sequence. It functions optimally at a temperature around 60-65 degrees Celsius, making it thermophilic. This property makes BstI particularly useful in DNA manipulations that require higher temperatures, such as polymerase chain reactions (PCR). In addition, the enzyme can work under a wide range of pH conditions and is not strongly influenced by ionic strength, making it versatile for various experimental settings.
Overall, Endonuclease BstI is an essential tool in molecular biology research, enabling precise and controlled cleavage of DNA at specific recognition sites, facilitating a wide range of DNA analysis and manipulation techniques.
Common Misspellings for ENDONUCLEASE BSTI
- wndonuclease bsti
- sndonuclease bsti
- dndonuclease bsti
- rndonuclease bsti
- 4ndonuclease bsti
- 3ndonuclease bsti
- ebdonuclease bsti
- emdonuclease bsti
- ejdonuclease bsti
- ehdonuclease bsti
- ensonuclease bsti
- enxonuclease bsti
- enconuclease bsti
- enfonuclease bsti
- enronuclease bsti
- eneonuclease bsti
- endinuclease bsti
- endknuclease bsti
- endlnuclease bsti
- endpnuclease bsti
Etymology of ENDONUCLEASE BSTI
The word "endonuclease BstI" has two main components:
1. Endonuclease: The word "endonuclease" is derived from "endo-" meaning "within, inside" and "nuclease" which refers to an enzyme that breaks down nucleic acids (DNA or RNA) into smaller components. Thus, "endonuclease" refers to an enzyme that cleaves within a nucleic acid molecule.
2. BstI: "BstI" is a specific type of endonuclease that is named after the bacterium Bacillus stearothermophilus strain. The letter "B" is often used to indicate the bacterial species, "st" refers to Bacillus stearothermophilus, and "I" is used to differentiate between different restriction enzymes derived from the same bacterium.
