ENDONUCLEASE BNAI Meaning and
Definition
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Endonuclease BnaI is a specific type II restriction endonuclease enzyme derived from the bacterium Bacillus natto. It is commonly used in molecular biology research for various applications, particularly in DNA manipulation and analysis.
Endonucleases are enzymes that catalyze the hydrolysis of phosphodiester bonds within a polynucleotide chain. BnaI, specifically, recognizes and cleaves double-stranded DNA at a particular nucleotide sequence, known as a recognition site, which consists of six base pairs. The recognition site for BnaI is GG↓TACC, where the symbol ↓ represents the cleavage site.
The enzyme BnaI belongs to the class of type II restriction endonucleases, which are characterized by their ability to cleave DNA at specific sites within their recognition sequences. BnaI is a tetrameric enzyme, with four subunits that work together to efficiently recognize and cleave DNA at its target site. It requires the presence of divalent metal ions, such as Mg2+, for its activity.
The cleavage by BnaI generates two DNA fragments with blunt ends, meaning that there are no overhanging nucleotides at the ends of the fragments. This property makes it suitable for a variety of applications in molecular biology, including DNA cloning, restriction mapping, genetic engineering, and DNA sequencing.
Overall, endonuclease BnaI is an important enzyme used in genetic research and manipulation due to its precise and specific DNA recognition and cleavage properties, facilitating the study and manipulation of DNA sequences.
Common Misspellings for ENDONUCLEASE BNAI
- wndonuclease bnai
- sndonuclease bnai
- dndonuclease bnai
- rndonuclease bnai
- 4ndonuclease bnai
- 3ndonuclease bnai
- ebdonuclease bnai
- emdonuclease bnai
- ejdonuclease bnai
- ehdonuclease bnai
- ensonuclease bnai
- enxonuclease bnai
- enconuclease bnai
- enfonuclease bnai
- enronuclease bnai
- eneonuclease bnai
- endinuclease bnai
- endknuclease bnai
- endlnuclease bnai
- endpnuclease bnai
Etymology of ENDONUCLEASE BNAI
The etymology of the word "Endonuclease BnaI" can be broken down as follows:
1. Endonuclease: The term "endonuclease" is derived from two Greek words. "Endo-" means "within" or "inside", and "nuclease" is derived from the word "nucleus", which refers to the cell nucleus. Thus, "endonuclease" refers to an enzyme that breaks down nucleic acids within the nucleus.
2. Bna: In this case, "Bna" is an abbreviation for Brassica napus, which is the scientific name for the oilseed rape plant. "Bna" is commonly used to denote enzymes or molecules that are derived from or associated with this particular plant species.
3. I: The letter "I" in "BnaI" represents the first restriction endonuclease isolated from Brassica napus.
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