SEPHAROSE CHROMATOGRAPHIES Meaning and
Definition
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Sepharose chromatography is a technique utilized in biochemistry and purification processes to separate and purify biomolecules with high specificity and efficiency. It involves the use of an engineered support matrix called Sepharose, which is composed of agarose beads cross-linked with a covalent linkage, such as cyanogen bromide.
The Sepharose matrix consists of a three-dimensional network of finely porous beads, which provides a large surface area for biomolecules to interact and bind selectively. The beads possess functional groups that can be customized to target specific molecules. These functional groups can be activated to form covalent bonds or ligands that have a high affinity towards the desired biomolecule to be purified.
The process of Sepharose chromatography involves passing a complex mixture of biomolecules through a column packed with the Sepharose beads. The biomolecules interact with the ligands on the beads through various interactions such as hydrophobic, electrostatic, or affinity interactions. This interaction causes the biomolecules of interest to bind to the Sepharose beads, while other unwanted impurities pass through the column unbound.
Once the mixture has been passed through the column, the bound biomolecules are then eluted from the Sepharose matrix using specific eluting agents or by altering the conditions, such as pH or ionic strength, to disrupt the binding interactions. This enables the purified biomolecule to be collected separately from the impurities.
Overall, Sepharose chromatography provides a versatile and efficient method for the purification and separation of biomolecules, making it a valuable tool in various areas of biochemistry, such as protein purification and isolation of nucleic acids.
Common Misspellings for SEPHAROSE CHROMATOGRAPHIES
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Etymology of SEPHAROSE CHROMATOGRAPHIES
The word "Sepharose" is derived from "Sephadex" and "agarose". "Sephadex" is a trademark for a range of cross-linked dextran beads used in chromatography, and "agarose" is a polysaccharide derived from seaweed that is commonly used as a matrix or support material for chromatography.
"Chromatographies" is the plural form of "chromatography", which is derived from two Greek words: "chroma" meaning "color" and "graphein" meaning "to write". The term "chromatography" was coined in the early 1900s by Russian botanist Mikhail Tsvet, who used it to describe a method for separating plant pigments based on their different colors.
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